LOCUS       sequence-150095-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5CZG (Tb427.10.7420)
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(499..517)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      513..560
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(735..740,741..757,758..763))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(790..808)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg cagagaaaac
       61 ttgtatttcc agggcatgag caagaacgag cgagatactt cttttaacaa gaatggctgt
      121 cttgtttttg tatcccgtct gtgggatctt gacaagcttg gaatgtttca tcacccagtt
      181 agtgccgaag aactgccgga ctaccacact gtgataaagc gtcctgttga cctctcttcc
      241 atacgcgatg ggattgaaaa gggcacttat gcaactgacg tggacgtaca gaacgacgtc
      301 gctcgcatga ttacgaacgc tttggagtac aatgcgaagg gcagcacgtg gtatcaggag
      361 gcaatgtcat ttcgtaaaac ttaccttgac cttgcacggc agtcgggact cgttgtggac
      421 tgatgacgaa gcttgcggcc gcactcgagg atccggctgc taacaaagcc cgaaaggaag
      481 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      541 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatccc gcaagaggcc
      601 cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat
      661 gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg
      721 tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa
      781 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      841 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      901 tctaa
//
