LOCUS       sequence-150099-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5E16 (PfPKG, PF14_0346)
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..36
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(574..592)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      588..635
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(810..815,816..832,833..838))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 agaaggagat ataccatgca tcatcatcat catcacagca gcggcagaga aaacttgtat
       61 ttccagggca gcaacgatga ctttacgggc gaagacagtc tgatggaaga tcatctggaa
      121 ctgcgtgaaa aactgtccga agatattgac atgatcaaaa ccagcctgaa aaacaatctg
      181 gtgtgctcta ccctgaatga taacgaaatt ctgacgctgt caaactacat gcaatttttc
      241 gtgttcaaat cgggcaacct ggttatcaaa cagggtgaaa aaggcagtta tttctttatc
      301 atcaactccg gtaaatttga tgtctacgtg aacgacaaaa aagttaaaac catgggcaaa
      361 ggtagctctt tcggcgaagc ggccctgatt cacaataccc agcgctctgc aacgattatc
      421 gctgaaaccg atggtacgct gtggggcgtc cagcgtagca cctttcgcgc gacgctgaaa
      481 caactgtcta accgttgatg acgaagcttg cggccgcact cgaggatccg gctgctaaca
      541 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      601 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      661 atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt
      721 gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg
      781 ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaa
//
