LOCUS       sequence-150100-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5E16 (PfPKG, PF14_0346)
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(473..493)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(506..523)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             533..555
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    570..594
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(594..613)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(595..613)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(697..716)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatcctc gagtgcggcc gcaagcttcg tcatcaacgg ttagacagtt
       61 gtttcagcgt cgcgcgaaag gtgctacgct ggacgcccca cagcgtacca tcggtttcag
      121 cgataatcgt tgcagagcgc tgggtattgt gaatcagggc cgcttcgccg aaagagctac
      181 ctttgcccat ggttttaact tttttgtcgt tcacgtagac atcaaattta ccggagttga
      241 tgataaagaa ataactgcct ttttcaccct gtttgataac caggttgccc gatttgaaca
      301 cgaaaaattg catgtagttt gacagcgtca gaatttcgtt atcattcagg gtagagcaca
      361 ccagattgtt tttcaggctg gttttgatca tgtcaatatc ttcggacagt ttttcacgca
      421 gttccagatg atcttccatc agactgtctt cgcccgtaaa gtcatcgttg ctgccctgga
      481 aatacaagtt ttctctgccg ctgctgtgat gatgatgatg atgcatggta tatctccttc
      541 ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
      601 gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg
      661 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
      721 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
      781 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
      841 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
      901 aggga
//
