LOCUS       sequence-150102-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5FEA (LdBRD19, LdBPK_363520.1)
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(562..580)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      576..623
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(798..803,804..820,821..826))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(853..871)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg cagagaaaac
       61 ttgtatttcc agggccaccc acggccgctg ccagccggca agcacgcgca tcgacagtcc
      121 ctagagacca tccccgaggt ggctgagctg tatcactgta tctacaagct ctacaatgag
      181 gaagagagct ctgtgtggtt tcgcgagccc gtcaacgcgc tggcgcagga aattttcacg
      241 tactacgacg tggtcaagtc acccatgagc ctgcgacaca ttctcgacaa cattgtaaag
      301 ggcgacacct attccaccgc cctacaggtg atggaagacg tggagctcat ctggaaaaac
      361 tgcattacct tcaacggtgc caactcgctc ttggccaccg aggcgggcaa gtgccgatcc
      421 gcgctagacc gaatccgtcg cgcctaccag gacgatcagc gcatcaccgt cgaggaggct
      481 gagtgatgac gaagcttgcg gccgcactcg aggatccggc tgctaacaaa gcccgaaagg
      541 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      601 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat cccgcaagag
      661 gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag
      721 gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa
      781 ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt
      841 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      901 tttctta
//
