LOCUS       sequence-150103-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5FEA (LdBRD19, LdBPK_363520.1)
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(451..471)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(484..501)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             511..533
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    548..572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(572..591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(573..591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..694)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atcctcgagt gcggccgcaa gcttcgtcat cactcagcct cctcgacggt
       61 gatgcgctga tcgtcctggt aggcgcgacg gattcggtct agcgcggatc ggcacttgcc
      121 cgcctcggtg gccaagagcg agttggcacc gttgaaggta atgcagtttt tccagatgag
      181 ctccacgtct tccatcacct gtagggcggt ggaataggtg tcgcccttta caatgttgtc
      241 gagaatgtgt cgcaggctca tgggtgactt gaccacgtcg tagtacgtga aaatttcctg
      301 cgccagcgcg ttgacgggct cgcgaaacca cacagagctc tcttcctcat tgtagagctt
      361 gtagatacag tgatacagct cagccacctc ggggatggtc tctagggact gtcgatgcgc
      421 gtgcttgccg gctggcagcg gccgtgggtg gccctggaaa tacaagtttt ctctgccgct
      481 gctgtgatga tgatgatgat gcatggtata tctccttctt aaagttaaac aaaattattt
      541 ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg
      601 gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      661 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
      721 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
      781 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
      841 cctactactg ggctgc
//
