LOCUS       sequence-150106-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear mCerulean2 (blue) fluorophore for use with 5' promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pME-H2B-mCerulean3
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     513..532
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gtactagttc
      121 atttatacag ttcatccatg cccagggtga ttccagcggc ggtcacaaat tccagcagca
      181 ccatgtggtc gcgcttctcg ttagggtcct tgctcagctt gctctgggtg ctcaggtagt
      241 ggttgtctgg cagcagcaca ggtccatctc cgataggggt gttctgctgg tagtggtcgg
      301 ccagctgcac gcttccgtcc tcgatgttgc agttcaggcc gaagttggcc ttgattccgt
      361 tcttctgctt gtcggcggtg atgtacacgt ttccgtggat ggcgttgtac tccagcttgt
      421 gtcccaggat gtttccgtcc tctttgaagt cgattccctt cagctcgatt ctgttgacca
      481 gggtgtcgcc ctcgaacttc acttcggctc tggtcttgta gttgccgtcg tccttgaaga
      541 agatggttct ttcctgcacg tatccctcag gcatggcgct cttgaagaaa tcgtgctgct
      601 tcatgtggtc agggtatctg gcgaagcact gcactcccca gctcagggtg gtgaccaggg
      661 taggccaagg cacaggcagc tttccggtgg tgcagatgaa cttcagggtc agctttccgt
      721 aggtggcgtc gccttcgccc tcgccggaca cgctgaactt gtgtccgttc acgtcgccgt
      781 ccagttccac caggataggc accacgccgg tgaacagttc ctcgccctta gacaccatag
      841 atcctccgcc gcttccgcct ccgcttcctc cgcccttgct gcttgtgtac ttggtcacgg
      901 cctttgttcc ctcagacacg gcgtgcttgg ccagctcgcc aggcagcagc agtctgacgg
      961 cgg
//
