LOCUS       sequence-150109-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5J3U (TgPKA, 583.m05422)
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg cagagaaaac
       61 ttgtatttcc agggcgtgta cgagaaagat gagggccaga aagagcaatt agagcgcatt
      121 ttacgccaat catttctgtt caacagtctg gacgaaaagg acctgaatac cgtcatctta
      181 gcgatgcagg aaaagaaaat tgaggcctca acgtgcctga tccgggaagg tgacgacggg
      241 gaatgtctgt atattgttca atccggagag ttaaactgct cgaaactgat cgatggtgag
      301 gagcgtgtag tgaaggtcgt tggtccaggg gacgcatttg gtgaactggc tttactgtac
      361 aatgcgcctc gcgctgcaac tgtgacaagc gtcagtgcgt gtgatctgtg ggaattaggg
      421 cgtgacacct tcaacgctat tgtcaaagat gcagccacaa aacgccggag tatgtatgac
      481 agcttcttaa agtccgttca catcctggat ggaatggatg cgtacgagcg tggcaaagtg
      541 gcggacgccc tgcgcaccga aatgttcact gatggtgcat atattgtacg tcagggggag
      601 ttaggcgacg tgttctatat cgtggaagaa ggttcggcgg tcgcaactaa gtcgtttggg
      661 cctggtcagc cgccgattga ggttaagaaa taccaagccg gtgattattt cggggaactg
      721 gcactgatca atgaggaacc acgtgctgcc aacgtgattg cacatggcat ttgcaaggta
      781 gcatgtctgg aacggaaaag cttcaaacgt ctgatgggta gtgtccagga tctgttatca
      841 aaaaaggctt ccgagtacaa acgcgaaagc gattgatgac gaa
//
