LOCUS       sequence-150113-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Floxed TagBFP2 (blue) fluorophore with stop codon for inducible 
            lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pME-loxP-mTagBFP2-stop-pA-loxP
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(95..128)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             135..836
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(898..917)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     952..971
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg cttaataact tcgtatagca tacattatac
      121 gaagttatgc caccatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
      181 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
      241 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
      301 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
      361 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
      421 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
      481 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
      541 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
      601 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga
      661 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
      721 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
      781 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aattaaggcg
      841 cgcctctaga actatagtga gtcgtattac gtagatccag acatgataag atacattgat
      901 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      961 gatgctattg ctttatttgt aaccattata agc
//
