LOCUS       sequence-150114-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Floxed TagBFP2 (blue) fluorophore with stop codon for inducible 
            lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pME-loxP-mTagBFP2-stop-pA-loxP
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    116..149
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    203..337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(240..259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     294..313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gtagtataac
      121 ttcgtataat gtatgctata cgaagttatt cccacacctc cccctgaacc tgaaacataa
      181 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      241 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      301 gtccaaactc atcaatgtat cttatcatgt ctggatctac gtaatacgac tcactatagt
      361 tctagaggcg cgccttaatt aagcttgtgc cccagtttgc tagggaggtc gcagtatctg
      421 gccactgcca cctcgtgctg ctcgacgtag gtctcgttgt tggcctcctt gattctttcc
      481 agtctgtagt ccacatagta gacgccaggc atcttgaggt tcttagcggg tttcttggat
      541 ctatatgtgg tcttggcgtt tgcgatcaga tggctcccgc ccacgagctt cagggccatg
      601 tcgtttctgc cttccaggcc gccgtcagcg gggtacagcg tctcggtgaa ggcctcccag
      661 ccgagtgttt tcttctgcat cacagggccg ttggatgtga agttcacccc tctgatcttg
      721 acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct gggtagcggt cagcacgccc
      781 ccgtcttcgt atgtggtgac tctctcccat gtgaagccct cagggaagga ctgcttgaag
      841 aagtcgggga tgccctgggt gtggttgatg aaggtcttgc tgccgtagag gaagctagta
      901 gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga ccaccttgat tctcatggtc
      961 tgggtgccct cgtagggctt gccttcgccc tcggatgtgc acttgaa
//
