LOCUS       sequence-150115-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5FAI (EMG1)
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             847..864
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg
       61 cagagaaaac ttgtatttcc agggcatggc cgcgcccagt gatggattca agcctcgtga
      121 acgaagcggt ggggagcagg cacaggactg ggatgctctg ccacccaagc ggccccgact
      181 aggggcagga aacaagatcg gaggccgtag gcttattgtg gtgctggaag gggccagtct
      241 ggagacagtc aaggtaggga agacatatga gctactcaac tgtgacaagc acaagtctat
      301 attgttgaag aatggacggg accctgggga agcgcggcca gatatcaccc accagagttt
      361 gctgatgctg atggatagtc ccctgaaccg agctggcttg ctacaggttt atatccatac
      421 acagaagaat gttctgattg aagtgaatcc ccagacccga attcccagaa cctttgaccg
      481 cttttgtggc ctcatggttc aacttttaca caagctcagt gttcgagcag ctgatggccc
      541 ccagaagctt ttgaaggtaa ttaagaatcc agtatcagat cactttccag ttggatgtat
      601 gaaagttggc acttcttttt ccatcccggt tgtcagtgat gtgcgtgagc tggtgcccag
      661 cagtgatcct attgtttttg tggtaggggc ctttgcccat ggcaaggtca gtgtggagta
      721 tacagagaag atggtgtcca tcagtaacta ccccctttct gctgccctca cctgtgcaaa
      781 acttaccaca gcctttgagg aagtatgggg ggtcatttga tgacgaagct tgcggccgca
      841 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaa
//
