LOCUS       sequence-150122-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5H9M (SIAH2)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(645..665)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttcgtcatc atgtagaaat agtaacattg attccaaggt tcccattatc tgcaaaaaga
      121 tgtgctatgg ctgtgtcgaa aacaaggcag tcgctgttca tgatggccgc agccacaccg
      181 tcatgaatcg aacggggcgt ggcctcccag gtcaatctcc gccggttccc attcaactcc
      241 agtctgtagg caaagttctc ggcttgcttg cgggtgccaa tgagcaggac gatggcaaaa
      301 aactgctggt ggccttcgta cttctcttgt ttctccagca ccagcatgaa gtgatggcca
      361 aaacatgact gcatcatcac ccagtcgaca gcccctggca agttaatgtc tgtagctaga
      421 aagacgatgt cttctccctg aagggtggta atgctcttgt gggcgtgcat gagatgggac
      481 atcacagctt ccagggaccc ctgccacttg caggaagcac caggacatgg gcaggagtag
      541 ggacggtatt cacatatgtc ttcatgttct ggtttctccg tatggtgcag ggtcagggaa
      601 cagcccgtgg tggcatactt acagggaaac aggactgccg aggcgccctg gaaatacaag
      661 ttttctgttc caccaatctg ttctctgtga gcctcaataa tatcgttatc ctccatgtcc
      721 aaatcttcag gggtctgatc agcttggatt ctaataccgt cgtacaagaa tcttaaggag
      781 tccatttcct taccctgtct tttagcgaac gcttccatca gccttcttaa aggagtggtc
      841 tttttgatct tgaa
//
