LOCUS       sequence-150125-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Full-length horseradish peroxidase (HRP) fused to the C-terminus of 
            the yeast mating protein Aga2P. Galactose-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: pCTCON2 Aga2P-HRP
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(54..334)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(355..374)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     474..496
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(534..552)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccgcaaactt tcaccaatgg accagaacta cctgtgaaat taataacaga catactccaa
       61 gctgcctttg tgtgcttaat cacgtatact cacgtgctca atagtcacca atgccctccc
      121 tcttggccct ctccttttct tttttcgacc gaattaattc ttaatcggca aaaaaagaaa
      181 agctccggat caagattgta cgtaaggtga caagctattt ttcaataaag aatatcttcc
      241 actactgcca tctggcgtca taactgcaaa gtacacatat attacgatgc tgttctatta
      301 aatgcttcct atattatata tatagtaatg tcgtgatcta tggtgcactc tcagtacaat
      361 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
      421 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
      481 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
      541 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cggatcgctt
      601 gcctgtaact tacacgcgcc tcgtatcttt taatgatgga ataatttggg aatttactct
      661 gtgtttattt atttttatgt tttgtatttg gattttagaa agtaaataaa gaaggtagaa
      721 gagttacgga atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt
      781 actttacata tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa
      841 cgataagtaa aatgtaaaat cacagga
//
