LOCUS       sequence-150127-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Full-length horseradish peroxidase (HRP) fused to the C-terminus of 
            the yeast mating protein Aga2P. Galactose-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: pCTCON2 Aga2P-HRP
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     95..118
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             450..461
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             462..488
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             495..515
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gcaaactgca taaccacttt actaatactt tcaacatttt cggtttgtat tacttcttat
       61 tcaaatgtaa taaaagtatc aacaaaaaat tgttaatata cctctatact ttaacgtcaa
      121 ggagaaaaaa ccccggatcg aattccctac ttcatacatt ttcaattaag atgcagttac
      181 ttcgctgttt ttcaatattt tctgttattg cttcagtttt agcacaggaa ctgacaacta
      241 tatgcgagca aatcccctca ccaactttag aatcgacgcc gtactctttg tcaacgacta
      301 ctattttggc caacgggaag gcaatgcaag gagtttttga atattacaaa tcagtaacgt
      361 ttgtcagtaa ttgcggttct cacccctcaa caactagcaa aggcagcccc ataaacacac
      421 agtatgtttt taaggacaat agctcgacga ttgaaggtag atacccatac gacgttccag
      481 actacgctct gcaggagaac ttgtactttc aaggtctgca ggctagtggt ggaggaggct
      541 ctggtggagg cggtagcgga ggcggagggt cggctagcca gcttactcca actttctacg
      601 ataactcttg tcctaacgtg agcaacatcg tgcgagatac tatcgtgaac gagttgcggt
      661 ctgatccgag gatagccgct tcaattttgc ggctgcattt ccacgattgt tttgtaaacg
      721 gctgtgatgc ttccatcctc ctcgacaata ccacctcttt caggactgaa aaggatgcct
      781 tcggta
//
