LOCUS       sequence-150131-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing Grb2 in E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: GST-PS-Grb2 (XSB363)
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..103
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     248..270
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
       61 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
      121 ttatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
      181 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      241 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
      301 cagatcgtca gtcagtcacg atgcggccgc tcgagtcgac ttagacgttc cggttcacgg
      361 gggtgacata attgcgggga aacatgccgg tctgcccgtg gcaagctcct ttccaccagt
      421 tggggtctga gttatccatg acatggataa aatctccccg gcggaagccc agctctccat
      481 cctcctgggg atcaaagtca aagagggcct ggacgtatgt cggctgctgt ggcacctgtt
      541 ctatgtcccg caggaatatc tgctggtttc tggagacaga tgtagatctg tgataatcca
      601 ccagctcatt caaagaattg aacttcacca cccagaggaa gtacttcccg gctccatctc
      661 ggagcacctt gaagtgctgc acatcgtttc caaacttgac agagagggag aagtccccag
      721 gagcgctctc actctctcgg ataagaaagg ccccatcgtg ccgctgtttg ctaagcattt
      781 cttctgcctt ggctctgggg attttgccaa aaaaccacgg atgtggtttc atttctatgt
      841 agttcttggg aatgaagccg tcttttccat taagctctgc cttgtaccag ttctgatcac
      901 attcttcgtt caaaaccttg aggatgtccc ccc
//
