LOCUS       sequence-150134-        2604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miRFP PAS domain fused to FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pC4-RHE-PAS  (FRB-PAS)
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..284
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             291..317
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          349..921
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(1053..1072)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1118..1173
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1172..1191)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        1527..1830
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1831..2034
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1985..2005
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2031..2055
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           2124..2163
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     regulatory      2178..2187
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctagaatcct ctggcatgag atgtggcatg aaggcctgga agaggcatct cgtttgtact
       61 ttggggaaag gaacgtgaaa ggcatgtttg aggtgctgga gcccttgcat gctatgatgg
      121 aacggggccc ccagactctg aaggaaacat cctttaatca ggcctatggt cgagatttaa
      181 tggaggccca agagtggtgc aggaagtaca tgaaatcagg gaatgtcaag gacctcctcc
      241 aagcctggga cctctattat catgtgttcc gacgaatctc aaagactagt tatccgtacg
      301 acgtaccaga ctacgcataa gaaaagtgag gatcctgaga acttcagggt gagtttgggg
      361 acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa
      421 gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga
      481 taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt
      541 tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc
      601 acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat
      661 cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga
      721 taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga
      781 aacaactaca tcctggtcat catcctgcct ttctctttat ggttacaatg atatacactg
      841 tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat
      901 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat
      961 tttggcaaag gattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     1021 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
     1081 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     1141 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     1201 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc catatgctgg
     1261 ctgccatgaa caaaggtggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     1321 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     1381 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     1441 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct
     1501 cgaggagcac gcgttcgagc tcgccccgtt acataactta cggtaaatgg cccgcctggc
     1561 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1621 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1681 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1741 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1801 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     1861 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     1921 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     1981 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     2041 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     2101 cgggaccgat ccagcctccg ggggatcttg gtggcgtgaa actcccgcac ctcttcggcc
     2161 agcgaattcc agaagccgcc accatggctt ctagaatggt agcaggtcat gcctctggca
     2221 gccccgcatt cgggaccgcc tctcattcga attgcgaaca tgaagagatc cacctcgccg
     2281 gctcgatcca gccgcatggc gcgcttctgg tcgtcagcga acatgatcat cgcgtcatcc
     2341 aggccagcgc caacgccgcg gaatttctga atctcggaag cgtactcggc gttccgctcg
     2401 ccgagatcga cggcgatctg ttgatcaaga tcctgccgca tctcgatccc accgccgaag
     2461 gcatgccggt cgcggtgcgc tgccggatcg gcaatccctc tacggagtac tgcggtctga
     2521 tgcatcggcc tccggaaggc gggctgatca tcgaactcga acgtgccggc ccgggaggcg
     2581 ggggaagcgg cggaggcggg tcct
//
