LOCUS       sequence-150135-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing CD45R0 (extracellular domain) His10 in Sf9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..643
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 gggcatcctt cccagccctg ggatgcctgc gctgctctcc ctcgtgagcc ttctctccgt
       61 gctgctgatg ggttgcgtag ctgaaaccgg tgaattcatg gacaaagact gcgaaatgaa
      121 gcgcaccacc ctggatagcc ctctgggcaa gctggaactg tctgggtgcg aacagggcct
      181 gcaccgtatc atcttcctgg gcaaaggaac atctgccgcc gacgccgtgg aagtgcctgc
      241 cccagccgcc gtgctgggcg gaccagagcc actgatgcag gccaccgcct ggctcaacgc
      301 ctactttcac cagcctgagg ccatcgagga gttccctgtg ccagccctgc accacccagt
      361 gttccagcag gagagcttta cccgccaggt gctgtggaaa ctgctgaaag tggtgaagtt
      421 cggagaggtc atcagctaca gccacctggc cgccctggcc ggcaatcccg ccgccaccgc
      481 cgccgtgaaa accgccctga gcggaaatcc cgtgcccatt ctgatcccct gccaccgggt
      541 ggtgcagggc gacctggacg tggggggcta cgagggcggg ctcgccgtga aagagtggct
      601 gctggcccac gagggccaca gactgggcaa gcctgggctg ggtggtggtt ctggtggttc
      661 tgtcgaccaa agcccaacac cttcccccac tgatgcctac cttaatgcct ctgaaacaac
      721 cactctgagc ccttctggaa gcgctgtcat ttcaaccaca acaatagcta ctactccatc
      781 taagccaaca tgtgatgaaa aatatgcaaa catcactgtg gattacttat ataacaagga
      841 aactaaatta tttacagcaa agctaaatgt taatgagaat gtggaatgtg gaaacaatac
      901 ttgcacaaac aatgaggtgc ataaccttac agaatgtaaa aatgcgtctg tttccatatc
      961 tcataattca tgtactgctc ctgataagac attaatatta gatgtgccac caggggt
//
