LOCUS       sequence-150136-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing CD45R0 (extracellular domain) His10 in Sf9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..76)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 cgcatgcctc gagactgcag gctctagatt cgaaagcggc cgcttagtga tggtgatggt
       61 gatggtgatg gtgatgacta gtcttagaat tataagatgt tgaatgatgt aaaataaagg
      121 gttctccagg atagtctcca ttgtgaaaat aggccttaaa agtgtagtct gttgaatatt
      181 gaagatcttt tacacggaaa tcgcaattct tatgcgactc atttctaacc agagtatttc
      241 cagcttcaac ttccaaatgg taacgttcat gggggccatt acggtccctg ggaggcctac
      301 acttgacatg catactatta tctgatgtca tggagacagt catgttccag acctggcttg
      361 gaggagcact tttagttgtg aaatgacaca ttgcagcact tccattacgt tgcacttttg
      421 caatgatgta ggcatgtaat gataaaacat atttcgtata aggttttaaa ttttgcaaat
      481 catatttgat gaggttttta tccagattga ggcaatcttt ttctgtctct tttatataac
      541 agagggtaaa attatgaaat gatctttgag ggggattcca ggtaattact ccttgatgtg
      601 cagcttcact tctacaaaaa ataatctgag gctctcctgg actcccaaaa tctgttttaa
      661 taattttact tgcgttagta aacttgtggt tattatagag tatttctgag tcacacttat
      721 actcatgttc gggttcaagg ttttctaatt taatttcttt attatcaaat atcatattac
      781 cacactgaaa tctgtaggta atattctgtg tatcacaagt aaaggtttca atatttt
//
