LOCUS       sequence-150138-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing His10 - SNAPf in E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: His10-PS-SNAPf (XSB522)
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     682..701
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        682..700
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             732..754
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             771..800
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             807..830
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
       61 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
      121 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
      181 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
      241 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
      301 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
      361 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
      421 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
      481 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
      541 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
      601 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc
      661 gagatctcga tcccgcgaaa ttaatacgac tcactatagg gagaccacaa cggtttccct
      721 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatggaagaa catcaccatc
      781 accatcacca tcaccatcac ggttctctgg aagttctgtt ccaggggccc ctgggatcca
      841 tggacaaaga ctgcgaaatg aagcgcacca ccctggatag ccctctgggc aagctggaac
      901 tgtctgggtg cgaacagggc ctgcaccgta tcatcttcct gggcaaagga acatctgccg
      961 ccga
//
