LOCUS       sequence-150144-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3s2-FL3
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..68
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             72..119
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     terminator      149..336
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(204..228)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     polyA_signal    356..437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     381..400
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(451..825)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVCVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(844..891)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 ctgcaggtcg acggatcccc gggttaatta acgctagcat gggtgactac aaggacgacg
       61 atgacaaggg agattacaaa gatgacgacg ataaggacta caaggacgac gatgacaagt
      121 ctagatgagg cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat
      181 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
      241 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
      301 gtatgaggtc gctcttattg accacacctc taccggcaga tccgctagga gatctgcagt
      361 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
      421 gctgcaataa acaagtttcg aggtcgagtg tcagtcctgc tcctcggcca cgaagtgcac
      481 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
      541 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
      601 gtacagctcg tccaggccgc gcacacacac ccaggccagg gtgttgtccg gcaccacctg
      661 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
      721 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
      781 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
      841 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacggtccgt
      901 tccaatgcac cgttcccggc cgcggaggct ggatcggtcc cggtgtcttc tatggaggtc
      961 aaaacagcgt ggatggcgtc tccaggcgat c
//
