LOCUS       sequence-150146-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3s2-FL3
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..401)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVCVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(420..467)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(564..588)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(585..788)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(614..634)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 caataaacaa gtttcgaggt cgagtgtcag tcctgctcct cggccacgaa gtgcacgcag
       61 ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc
      121 atggccggcc cggaggcgtc ccggaagttc gtggacacga cctccgacca ctcggcgtac
      181 agctcgtcca ggccgcgcac acacacccag gccagggtgt tgtccggcac cacctggtcc
      241 tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc
      301 acgaagtccc gggagaaccc gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg
      361 cgcgcggtga gcaccggaac ggcactggtc aacttggcca tggtttagtt cctcaccttg
      421 tcgtattata ctatgccgat atactatgcc gatgattaat tgtcaacacg gtccgttcca
      481 atgcaccgtt cccggccgcg gaggctggat cggtcccggt gtcttctatg gaggtcaaaa
      541 cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct gcttatatag
      601 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
      661 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
      721 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
      781 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
      841 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
      901 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
      961 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     1021 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
//
