LOCUS       sequence-150148-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3s2-HA3
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..69
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             73..99
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             106..132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      165..352
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(220..244)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     polyA_signal    372..453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     397..416
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacgctagca tgtacccata cgatgttcct
       61 gactatgcgg gctacccgta tgacgtgccg gactatgcag gagcctatcc ttatgacgtt
      121 ccagattacg ctggttctag atgaggcgcg ccacttctaa ataagcgaat ttcttatgat
      181 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
      241 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt
      301 tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg
      361 ctaggagatc tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
      421 tttgtaacca ttataagctg caataaacaa gtttcgaggt cgagtgtcag tcctgctcct
      481 cggccacgaa gtgcacgcag ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg
      541 gctgctcgcc gatctcggtc atggccggcc cggaggcgtc ccggaagttc gtggacacga
      601 cctccgacca ctcggcgtac agctcgtcca ggccgcgcac acacacccag gccagggtgt
      661 tgtccggcac cacctggtcc tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca
      721 caccggcgaa gtcgtcctcc acgaagtccc gggagaaccc gagccggtcg gtccagaact
      781 cgaccgctcc ggcgacgtc
//
