LOCUS       sequence-150149-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3s2-HA3
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..406)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVCVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(425..472)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(569..593)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(590..793)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(619..639)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        794..1097
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agctgcaata aacaagtttc gaggtcgagt gtcagtcctg ctcctcggcc acgaagtgca
       61 cgcagttgcc ggccgggtcg cgcagggcga actcccgccc ccacggctgc tcgccgatct
      121 cggtcatggc cggcccggag gcgtcccgga agttcgtgga cacgacctcc gaccactcgg
      181 cgtacagctc gtccaggccg cgcacacaca cccaggccag ggtgttgtcc ggcaccacct
      241 ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt
      301 cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc gctccggcga
      361 cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatggtt tagttcctca
      421 ccttgtcgta ttatactatg ccgatatact atgccgatga ttaattgtca acacggtccg
      481 ttccaatgca ccgttcccgg ccgcggaggc tggatcggtc ccggtgtctt ctatggaggt
      541 caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga gctctgctta
      601 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
      661 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
      721 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
      781 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
      841 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
      901 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
      961 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     1021 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     1081 ccgtaagtta tgtaacggtt t
//
