LOCUS       sequence-150156-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5I3L (DPF3)
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(406..426)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(439..456)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             466..488
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    503..527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(527..546)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(528..546)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(630..649)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttcgtca
       61 tcatttgagc agttcccagc ataagtggca gctccagctt ccttctgggg gctcagccac
      121 cgggggattt aaacagtaca tgtgatagcc tcggtcacag tcatcgcaga agagtagctg
      181 gtcatcattc tctgaggtcc cacagaggat acaggatttg cactctatgc actgccactt
      241 gtaggtcttg acagcctcgg tcatgttcag ggtaaactgc aggcaggttg ggtgaccaga
      301 gcgtccacag tctgcgcagg acaccagctc ttcaggccgc ccactcttct tgttcatgtt
      361 ggagcccccc aagcagaagt cacagtagtt attgggaatg actgtgccct ggaaatacaa
      421 gttttctctg ccgctgctgt gatgatgatg atgatgcatg gtatatctcc ttcttaaagt
      481 taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc
      541 gtattaattt cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat
      601 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
      661 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
      721 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
      781 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataaggga
//
