LOCUS       sequence-150159-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5J39 (TDRKH)
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             675..692
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(745..763)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      759..806
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg cagagaaaac
       61 ttgtatttcc agggcgaagt ctacgtttct gcttctgagc accctaacca cttctggatc
      121 cagatcgttg gctcccgcag cctgcaattg gataagcttg tcaatgagat gacccagcac
      181 tatgagaata gtgtgcctga agacttgact gtgcatgtag gagacattgt agcagcacct
      241 ttacctacaa atggttcctg gtatcgagcc cgggtcctcg gcaccttgga gaatgggaac
      301 ttggacctct attttgttga ctttggagat aatggagatt gcccactgaa ggacctcagg
      361 gctctcagga gtgacttcct aagccttcca tttcaagcaa tagaatgtag tctggcacgg
      421 attgctccct caggtgacca gtgggaagag gaagctttgg atgagtttga tagactcact
      481 cattgtgctg actggaagcc tctggtagcc aagatctcta gctatgtcca gactgggatc
      541 tcaacttggc caaagatcta cttatatgat actagcaatg ggaagaaact tgatattggg
      601 ctagaattag tacacaaagg atacgcaatt gagcttcctg aagactgatg acgaagcttg
      661 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      721 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      781 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
      841 ggacgcgccc tgtagcggcg cattaagcgc ggcggg
//
