LOCUS       sequence-150160-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5J39 (TDRKH)
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(650..670)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(683..700)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg
       61 gccgcaagct tcgtcatcag tcttcaggaa gctcaattgc gtatcctttg tgtactaatt
      121 ctagcccaat atcaagtttc ttcccattgc tagtatcata taagtagatc tttggccaag
      181 ttgagatccc agtctggaca tagctagaga tcttggctac cagaggcttc cagtcagcac
      241 aatgagtgag tctatcaaac tcatccaaag cttcctcttc ccactggtca cctgagggag
      301 caatccgtgc cagactacat tctattgctt gaaatggaag gcttaggaag tcactcctga
      361 gagccctgag gtccttcagt gggcaatctc cattatctcc aaagtcaaca aaatagaggt
      421 ccaagttccc attctccaag gtgccgagga cccgggctcg ataccaggaa ccatttgtag
      481 gtaaaggtgc tgctacaatg tctcctacat gcacagtcaa gtcttcaggc acactattct
      541 catagtgctg ggtcatctca ttgacaagct tatccaattg caggctgcgg gagccaacga
      601 tctggatcca gaagtggtta gggtgctcag aagcagaaac gtagacttcg ccctggaaat
      661 acaagttttc tctgccgctg ctgtgatgat gatgatgatg catggtatat ctccttctta
      721 a
//
