LOCUS       sequence-150162-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5J3E (THYN1)
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             783..800
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(853..871)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg cagagaaaac
       61 ttgtatttcc agggcatgtc gagaccccgg aagaggctgg ctgggacttc tggttcagac
      121 aagggactat caggaaaacg caccaaaact gagaactcag gtgaggcatt agctaaagtg
      181 gaggactcca accctcagaa gacttcagcc actaaaaact gtttgaagaa tctaagcagc
      241 cactggctga tgaagtcaga gccagagagc cgcctagaga aaggtgtaga tgtgaagttc
      301 agcattgagg atctcaaagc acagcccaaa cagacaacat gctgggatgg tgttcgtaac
      361 taccaggctc ggaacttcct tagagccatg aagctgggag aagaagcctt cttctaccat
      421 agcaactgca aagagccagg catcgcagga ctcatgaaga tcgtgaaaga ggcttaccca
      481 gaccacacac agtttgagaa aaacaatccc cattatgacc catctagcaa agaggacaac
      541 cctaagtggt ccatggtgga tgtacagttt gttcggatga tgaaacgttt cattcccctg
      601 gctgagctca aatcctatca tcaagctcac aaagctactg gtggcccctt aaaaaatatg
      661 gttctcttca ctcgccagag attatcaatc cagcccctga cccaggaaga gtttgatttt
      721 gttttgagcc tggaggaaaa ggaaccaagt taatgatgac gaagcttgcg gccgcactcg
      781 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
      841 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      901 tgaggg
//
