LOCUS       sequence-150163-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5J3E (THYN1)
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(746..766)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(779..796)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             806..828
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    843..867
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttc
       61 gtcatcatta acttggttcc ttttcctcca ggctcaaaac aaaatcaaac tcttcctggg
      121 tcaggggctg gattgataat ctctggcgag tgaagagaac catatttttt aaggggccac
      181 cagtagcttt gtgagcttga tgataggatt tgagctcagc caggggaatg aaacgtttca
      241 tcatccgaac aaactgtaca tccaccatgg accacttagg gttgtcctct ttgctagatg
      301 ggtcataatg gggattgttt ttctcaaact gtgtgtggtc tgggtaagcc tctttcacga
      361 tcttcatgag tcctgcgatg cctggctctt tgcagttgct atggtagaag aaggcttctt
      421 ctcccagctt catggctcta aggaagttcc gagcctggta gttacgaaca ccatcccagc
      481 atgttgtctg tttgggctgt gctttgagat cctcaatgct gaacttcaca tctacacctt
      541 tctctaggcg gctctctggc tctgacttca tcagccagtg gctgcttaga ttcttcaaac
      601 agtttttagt ggctgaagtc ttctgagggt tggagtcctc cactttagct aatgcctcac
      661 ctgagttctc agttttggtg cgttttcctg atagtccctt gtctgaacca gaagtcccag
      721 ccagcctctt ccggggtctc gacatgccct ggaaatacaa gttttctctg ccgctgctgt
      781 gatgatgatg atgatgcatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga
      841 ggggaattgt tatccgctca caattcccc
//
