LOCUS       sequence-150168-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3a2-PK3
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..80
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             84..125
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             126..167
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      197..384
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(252..276)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     polyA_signal    404..485
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     429..448
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(615..634)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ctgcaggtcg acggatcccc gggttaatta acgctagcgg taagcctatt cctaaccctc
       61 ttttgggtct cgattctaca ggtggaaaac caatccccaa cccactcctc ggccttgact
      121 caactggcaa acccatacca aatcctttac ttggattaga tagtacctct agatgaggcg
      181 cgccacttct aaataagcga atttcttatg atttatgatt tttattatta aataagttat
      241 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
      301 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt atgaggtcgc
      361 tcttattgac cacacctcta ccggcagatc cgctaggaga tctgcagtga aaaaaatgct
      421 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      481 aagttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
      541 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
      601 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
      661 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
      721 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
      781 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
      841 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
      901 aatgggcagg tagccggatc aagcgta
//
