LOCUS       sequence-150169-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3a2-PK3
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(85..132)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(229..253)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(250..453)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(279..299)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        454..757
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(831..850)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(832..850)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat ccagcttttt gcaaaagcct
       61 agccatggtt tagttcctca ccttgtcgta ttatactatg ccgatatact atgccgatga
      121 ttaattgtca acacggtccg ttccaatgca ccgttcccgg ccgcggaggc tggatcggtc
      181 ccggtgtctt ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt
      241 cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
      301 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
      361 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
      421 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
      481 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
      541 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
      601 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
      661 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
      721 tcagccaggc gggccattta ccgtaagtta tgtaacggtt taaacgagct cgaattcatc
      781 gatgatatca gatccactag tggcctatgc ggccgcggat ctgccggtct ccctatagtg
      841 agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc a
//
