LOCUS       sequence-150170-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3a2-HA3
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..77
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             81..107
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             114..140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      173..360
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(228..252)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     polyA_signal    380..461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     405..424
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(591..610)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 cttcgtacgc tgcaggtcga cggatccccg ggttaattaa cgctagcatg tacccatacg
       61 atgttcctga ctatgcgggc tacccgtatg acgtgccgga ctatgcagga gcctatcctt
      121 atgacgttcc agattacgct ggttctagat gaggcgcgcc acttctaaat aagcgaattt
      181 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt
      241 ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta
      301 ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca cctctaccgg
      361 cagatccgct aggagatctg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
      421 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tcgaacccca gagtcccgct
      481 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
      541 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
      601 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
      661 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
      721 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
      781 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
      841 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
      901 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
      961 gatgacagga gatcctgccc c
//
