LOCUS       sequence-150171-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3a2-HA3
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(83..130)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(227..251)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(248..451)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(277..297)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        452..755
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(829..848)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(830..848)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc agctttttgc aaaagcctag
       61 ccatggttta gttcctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt
      121 aattgtcaac acggtccgtt ccaatgcacc gttcccggcc gcggaggctg gatcggtccc
      181 ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca
      241 ctaaacgagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
      301 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
      361 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
      421 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
      481 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
      541 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
      601 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
      661 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
      721 agccaggcgg gccatttacc gtaagttatg taacggttta aacgagctcg aattcatcga
      781 tgatatcaga tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag
      841 tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga
      901 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactga
//
