LOCUS       sequence-150174-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3a2-Myc13
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..553
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      616..803
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(671..695)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     polyA_signal    823..904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     848..867
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtacgctgca ggtcgacgga tccccgggtt aattaacgct agcgcgatta acggtgaaca
       61 aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag aagaagactt
      121 gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa
      181 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
      241 cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct
      301 aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg
      361 actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat
      421 ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact tgaacggact
      481 cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc
      541 cgaggaagac ttgaacggtg aacaaaaatt aatcaatcag tacaagtcta gatgaggcgc
      601 gccacttcta aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata
      661 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta
      721 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagta tgaggtcgct
      781 cttattgacc acacctctac cggcagatcc gctaggagat ctgcagtgaa aaaaatgctt
      841 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
      901 agttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc
      961 gctgcgaatc gggagcgg
//
