LOCUS       sequence-150180-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3s2-Myc13
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..552
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      615..802
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(670..694)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     polyA_signal    822..903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     847..866
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta attaacgcta gcgcgattaa cggtgaacaa
       61 aagctaatct ccgaggaaga cttgaacggt gaacaaaaat taatctcaga agaagacttg
      121 aacggactcg acggtgaaca aaagttgatt tctgaagaag atttgaacgg tgaacaaaag
      181 ctaatctccg aggaagactt gaacggtgaa caaaaattaa tctcagaaga agacttgaac
      241 ggactcgacg gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta
      301 atctccgagg aagacttgaa cggtgaacaa aaattaatct cagaagaaga cttgaacgga
      361 ctcgacggtg aacaaaagtt gatttctgaa gaagatttga acggtgaaca aaagctaatc
      421 tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag aagaagactt gaacggactc
      481 gacggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc
      541 gaggaagact tgaacggtga acaaaaatta atcaatcagt acaagtctag atgaggcgcg
      601 ccacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa
      661 aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat
      721 tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc
      781 ttattgacca cacctctacc ggcagatccg ctaggagatc tgcagtgaaa aaaatgcttt
      841 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
      901 gtttcgaggt cgagtgtcag tcctgctcct cggccacgaa gtgcacgcag ttgccggccg
      961 ggtcgcgcag ggcgaact
//
