LOCUS       sequence-150181-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3s2-Myc13
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..405)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVCVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(424..471)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(568..592)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(589..792)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(618..638)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctgcaataa acaagtttcg aggtcgagtg tcagtcctgc tcctcggcca cgaagtgcac
       61 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
      121 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
      181 gtacagctcg tccaggccgc gcacacacac ccaggccagg gtgttgtccg gcaccacctg
      241 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
      301 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
      361 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
      421 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacggtccgt
      481 tccaatgcac cgttcccggc cgcggaggct ggatcggtcc cggtgtcttc tatggaggtc
      541 aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctctgcttat
      601 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
      661 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
      721 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
      781 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
      841 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
      901 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
      961 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tggg
//
