LOCUS       sequence-150195-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCNK2
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             834..854
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             855..884
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             885..908
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatgcccac agtgcttgct tcccgggtgg agagtgacac gaccattaat gttatgaaat
       61 ggaagacggt ctccacgata ttcctggtgg ttgtcctcta tctgatcatc ggagccaccg
      121 tgttcaaagc attggagcag cctcatgaga tttcacagag gaccaccatt gtgatccaga
      181 agcaaacatt catatcccaa cattcctgtg tcaattcgac ggagctggat gaactcattc
      241 agcaaatagt ggcagcaata aatgcaggga ttataccgtt aggaaacacc tccaatcaaa
      301 tcagtcactg ggatttggga agttccttct tctttgctgg cactgttatt acaaccatag
      361 gatttggaaa catctcacca cgcacagaag gcggcaaaat attctgtatc atctatgcct
      421 tactgggaat tcccctcttt ggttttctct tggctggagt tggagatcag ctaggcacca
      481 tatttggaaa aggaattgcc aaagtggaag atacgtttat taagtggaat gttagtcaga
      541 ccaagattcg catcatctca acaatcatat ttatactatt tggctgtgta ctctttgtgg
      601 ctctgcctgc gatcatattc aaacacatag aaggctggag tgccctggac gccatttatt
      661 ttgtggttat cactctaaca actattggat ttggtgacta cgttgcaggt ggatccgata
      721 ttgaatatct ggacttctat aagcctgtcg tgtggttctg gatccttgta gggcttgctt
      781 actttgctgc tgtcctgagc atgattggag attggctccg agtgatatct gcagagaacc
      841 tctacttcca atcgcaccat catcaccatc accatcacca ccatgattac aaggatgacg
      901 acgataagtg aggatccgaa ttcgagctcc gtcgacaagc t
//
