LOCUS       sequence-150196-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCNK2
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..90)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(91..120)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(121..141)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tggctgatta tgatcctcta gtacttctcg acaagcttgt cgacggagct cgaattcgga
       61 tcctcactta tcgtcgtcat ccttgtaatc atggtggtga tggtgatggt gatgatggtg
      121 cgattggaag tagaggttct ctgcagatat cactcggagc caatctccaa tcatgctcag
      181 gacagcagca aagtaagcaa gccctacaag gatccagaac cacacgacag gcttatagaa
      241 gtccagatat tcaatatcgg atccacctgc aacgtagtca ccaaatccaa tagttgttag
      301 agtgataacc acaaaataaa tggcgtccag ggcactccag ccttctatgt gtttgaatat
      361 gatcgcaggc agagccacaa agagtacaca gccaaatagt ataaatatga ttgttgagat
      421 gatgcgaatc ttggtctgac taacattcca cttaataaac gtatcttcca ctttggcaat
      481 tccttttcca aatatggtgc ctagctgatc tccaactcca gccaagagaa aaccaaagag
      541 gggaattccc agtaaggcat agatgataca gaatattttg ccgccttctg tgcgtggtga
      601 gatgtttcca aatcctatgg ttgtaataac agtgccagca aagaagaagg aacttcccaa
      661 atcccagtga ctgatttgat tggaggtgtt tcctaacggt ataatccctg catttattgc
      721 tgccactatt tgctgaatga gttcatccag ctccgtcgaa ttgacacagg aatgttggga
      781 tatgaatgtt tgcttctgga tcacaatggt ggtcctctgt gaaatctcat gaggctgctc
      841 caatgctttg aa
//
