LOCUS       sequence-150200-         567 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from 
            a single open reading frame..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Rose T, Jaepel J, Hubener M, Bonhoeffer T
  TITLE     Cell-specific restoration of stimulus preference after monocular 
            deprivation in the visual cortex.
  JOURNAL   Science. 2016 Jun 10;352(6291):1319-22. doi: 
            10.1126/science.aad3358.
  PUBMED    27284193
REFERENCE   2  (bases 1 to 567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-10- 10"; volume: "352"; 
            issue: "6291"; pages: "1319-22"
COMMENT     Sequence Label: pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..567
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(82..115)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acttcgtata ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta
       61 ttagcaatat gaaacctctt aataacttcg tatagcatac attatacgaa gttatactag
      121 tgcggccgct tacttcgctg tcatcatttg tacaaactct tcgtagttta cctgaccatc
      181 cccatcgatg tctgcttccc tgatcatttc atcaacctct tcatctgtta acttctctcc
      241 aaggtttgtc atcacgtggc gaagctctgc tgcactgatg tagccattgc catccttatc
      301 aaacacaccg aacgcttctc taatttcttc ttccgtgtcc ctgtatttca tttttcttgc
      361 catcattgtc aggaactcag ggaagtcgat tgtgccgtca ccgtcggcat ctacttcatt
      421 gatcatgtcc tgcagctctg cttctgtggg gttctgcccc agagaccgca tcaccgtccc
      481 cagctccttg gttgttattg tcccatcccc gtccttgtca aatagggaga attcctcttt
      541 aaattctgcg atctgctctt cagtcag
//
