LOCUS       sequence-150203-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: JMJD1CA-c089
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(84..107)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(108..125)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(126..146)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc tcacttatcg tcgtcatcct tgtaatcatg gtggtgatga
      121 tggtgcgatt ggaagtagag gttctctgcc aaaagtctca gttcctgtgt taaatgaaat
      181 gactctacaa gatgttctgg agacacaaaa tcttcagtta cctgaataca gctgtgaaaa
      241 ttctgaacct gatgaagtgc tcccgctggc aaaacaatag catcaccaag gaactgaata
      301 agagtacagg ttctgactcc atattcttca agcagccttt gacggagctt tttgttcaca
      361 taccaacttt ggtcacgtat tggatcatgt tctggtagaa cttcaaggcc ttgttctttt
      421 gaaatctttt gaagaaattc ccttatcttg tcaacatctt tcccagcata aatatgccac
      481 agagcaccag gtatttcact tgagtccttc aatcttttcc ttaaaatgtc atccaaatct
      541 tcttcctcaa atttcttgag aattcctgct tttgagagaa tgccatttcc ttttgctatg
      601 ccaacataaa ctagtatatt tacaacatca gaaacttcaa tatggagatt tgttgttcct
      661 atatcatgat ctttagcagc aactacacca taggcactgc acaacctggg tcctagatca
      721 ggacgtacaa aaaatcctgg caaatgagag gccaaattga attttccttc tggattacaa
      781 tattctggca atggcagact ttttaaa
//
