LOCUS       sequence-150210-         428 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from 
            a single open reading frame..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Rose T, Jaepel J, Hubener M, Bonhoeffer T
  TITLE     Cell-specific restoration of stimulus preference after monocular 
            deprivation in the visual cortex.
  JOURNAL   Science. 2016 Jun 10;352(6291):1319-22. doi: 
            10.1126/science.aad3358.
  PUBMED    27284193
REFERENCE   2  (bases 1 to 428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-10- 10"; volume: "352"; 
            issue: "6291"; pages: "1319-22"
COMMENT     Sequence Label: pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..428
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(23..56)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(107..140)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agcgcagtcg aattcagatc ttataacttc gtataggata ctttatacga agttatgcag
       61 aatggtagct ggattgtagc tgctattagc aatatgaaac ctcttaataa cttcgtatag
      121 catacattat acgaagttat actagtgcgg ccgcttactt cgctgtcatc atttgtacaa
      181 actcttcgta gtttacctga ccatccccat cgatgtctgc ttccctgatc atttcatcaa
      241 cctcttcatc tgttaacttc tctccaaggt ttgtcatcac gtggcgaagc tctgctgcac
      301 tgatgtagcc attgccatcc ttatcaaaca caccgaacgc ttctctaatt tcttcttccg
      361 tgtccctgta tttcattttt cttgccatca ttgtcaggaa ctcagggaag tcgattgtgc
      421 cgtcaccg
//
