LOCUS       sequence-150212-         586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from 
            a single open reading frame..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Rose T, Jaepel J, Hubener M, Bonhoeffer T
  TITLE     Cell-specific restoration of stimulus preference after monocular 
            deprivation in the visual cortex.
  JOURNAL   Science. 2016 Jun 10;352(6291):1319-22. doi: 
            10.1126/science.aad3358.
  PUBMED    27284193
REFERENCE   2  (bases 1 to 586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-10- 10"; volume: "352"; 
            issue: "6291"; pages: "1319-22"
COMMENT     Sequence Label: pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(26..59)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(110..143)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      168..177
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccagaggttg attatcgata agcttataac ttcgtatagc atacattata cgaagttatt
       61 ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaaa taacttcgta
      121 taggatactt tatacgaagt tatctcgaga ctagtaccgg tcctagggcc accatggtgt
      181 ctaagggcga agagctgatc aaggaaaata tgcgtatgaa ggtggtcatg gaaggttcgg
      241 tcaacggcca ccaattcaaa tgcacaggtg aaggagaagg caatccgtac atgggaactc
      301 aaaccatgag gatcaaagtc atcgagggag gacccctgcc atttgccttt gacattcttg
      361 ccacgtcgtt catgtatggc agccgtactt ttatcaagta cccgaaaggc attcctgatt
      421 tctttaaaca gtcctttcct gagggtttta cttgggaaag agttacgaga tacgaagatg
      481 gtggagtcgt caccgtcatg caggacacca gccttgagga tggctgtctc gtttaccacg
      541 tccaagtcag aggggtaaac tttccctcca atggtcccgt gatgca
//
