LOCUS       sequence-150219-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for expressing CD3z-SNAPf-ybbR in E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton
            J, Rosen MK, Vale RD
  TITLE     Phase separation of signaling molecules promotes T cell receptor 
            signal transduction.
  JOURNAL   Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. 
            Epub 2016 Apr 7.
  PUBMED    27056844
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad9964"; journalName: "Science"; date: 
            "2016-04-29- 29"; volume: "352"; issue: "6285"; pages: "595-9"
COMMENT     Sequence Label: His10-CD3z-SNAPf-ybbR (XSB507)
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(90..137)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     133..151
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(204..221)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(285..827)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
ORIGIN
        1 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
       61 gccaatccgg atatagttcc tcctttcagc aaaaaacccc tcaagacccg tttagaggcc
      121 ccaaggggtt atgctagtta ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg
      181 gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcttaa
      241 ctagtttaag ccagtttaga agcgatgaat tccagagagt caccacccag cccaggcttg
      301 cccagtctgt ggccctcgtg ggccagcagc cactctttca cggcgagccc gccctcgtag
      361 ccccccacgt ccaggtcgcc ctgcaccacc cggtggcagg ggatcagaat gggcacggga
      421 tttccgctca gggcggtttt cacggcggcg gtggcggcgg gattgccggc cagggcggcc
      481 aggtggctgt agctgatgac ctctccgaac ttcaccactt tcagcagttt ccacagcacc
      541 tggcgggtaa agctctcctg ctggaacact gggtggtgca gggctggcac agggaactcc
      601 tcgatggcct caggctggtg aaagtaggcg ttgagccagg cggtggcctg catcagtggc
      661 tctggtccgc ccagcacggc ggctggggca ggcacttcca cggcgtcggc ggcagatgtt
      721 cctttgccca ggaagatgat acggtgcagg ccctgttcgc acccagacag ttccagcttg
      781 cccagagggc tatccagggt ggtgcgcttc atttcgcagt ctttgtcaga accaccagaa
      841 ccacctcttg gtggcagggc ctgcatgtga agggcgtcgt aggtgtcctt ggtggctgta
      901 ctgagaccct ggtaaaggcc atcgtgcccc ttgcccctcc ggcgctcgcc tttcatccca
      961 atctcactgt aggcctccgc catcttatct ttctgcagtt cattgtacag gccttcctga
     1021 gggttcttcc ttctctgcgg ctttcccc
//
