LOCUS       sequence-150220-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Indicator of caspase-3-like protease activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Zhang J, Wang X, Cui W, Wang W, Zhang H, Liu L, Zhang Z, Li Z, Ying 
            G, Zhang N, Li B
  TITLE     Visualization of caspase-3-like activity in cells using a 
            genetically encoded fluorescent biosensor activated by protein 
            cleavage.
  JOURNAL   Nat Commun. 2013;4:2157. doi: 10.1038/ncomms3157.
  PUBMED    23857461
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2157. doi"; pages: " 
            10.1038/ncomms3157"
COMMENT     Sequence Label: pCDH-puro-CMV-RC3AI
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(584..602)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agcagagctc gttttgtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gattctagag ctagcgaatt gccaccatgt acccctacga cgtgcccgac
      121 tacgccatca agatcgccac caggaagtac ctgggcaagc agaacgtgta cgacatcggc
      181 gtggagaggg accacaactt cgccctgaag aacggcttca tcgccagcaa ctgcttcaac
      241 gaattcatgg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
      301 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      361 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
      421 gaacagtacg aacgcgccga gggccgccac tccaccgacg aggtggacgg catcatcaag
      481 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      541 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      601 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      661 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      721 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      781 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaactt
//
