LOCUS       sequence-150222-         247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for production of MBP-3C site-His6-TEV 
            site-passenger protein fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Raran-Kurussi S, Waugh DS
  TITLE     A dual protease approach for expression and affinity purification of
            recombinant proteins.
  JOURNAL   Anal Biochem. 2016 Apr 19;504:30-37. doi: 10.1016/j.ab.2016.04.006.
  PUBMED    27105777
REFERENCE   2  (bases 1 to 247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2016-04-19"; volume: "504:30-37. doi"; pages: " 
            10.1016/j.ab.2016.04.006"
COMMENT     Sequence Label: pSRK2721
FEATURES             Location/Qualifiers
     source          1..247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(18..120)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
       61 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttgc gtgccgatca
      121 acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga
      181 tttatttatt ctgcgaagtg atcttccgtc acaggtattt attcggcgca aagtgcgtcg
      241 ggtgatc
//
