LOCUS       sequence-150225-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Indicator of caspase-3-like protease activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Zhang J, Wang X, Cui W, Wang W, Zhang H, Liu L, Zhang Z, Li Z, Ying 
            G, Zhang N, Li B
  TITLE     Visualization of caspase-3-like activity in cells using a 
            genetically encoded fluorescent biosensor activated by protein 
            cleavage.
  JOURNAL   Nat Commun. 2013;4:2157. doi: 10.1038/ncomms3157.
  PUBMED    23857461
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2157. doi"; pages: " 
            10.1038/ncomms3157"
COMMENT     Sequence Label: pCDH-puro-CMV-RC3AI
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..630)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(114..134)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     611..630
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac tcaggcaccg ggcttgcggg tcatgcacca
       61 ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta
      121 gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc
      181 cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga
      241 gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc
      301 ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat
      361 ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc
      421 gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc
      481 ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg
      541 gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc
      601 gaggcgcacc gtgggcttgt actcggtcat ctagcgtagg cgccggtcac agcttggatc
      661 tgtaacggcg cagaacagaa aacgaaacaa agacgtagag ttgagcaagc agggtcaggc
      721 aaagcgtgga agagccggct gagtctaggt aggctcccaa gggaagcgcc ggacaaaggc
      781 ccggtctcga acctgagctt ttaaaactta ccttagacgg cggacgccag tttcaggaag
      841 gcaacaa
//
