LOCUS       sequence-150227-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 
            site for cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim JS
  TITLE     Genome-wide analysis reveals specificities of Cpf1 endonucleases in 
            human cells.
  JOURNAL   Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609.
  PUBMED    27272384
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609."
COMMENT     Sequence Label: pU6-As-crRNA
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     206..223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(401..649)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(459..478)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(629..649)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        764..868
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
       61 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      241 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagga agacaactag
      301 tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaatctag aaaaaaggag
      361 acggatggat cccgtctcaa tctacaagag tagaaattac ggtgtttcgt cctttccaca
      421 agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata tgatagtcca
      481 ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt ctacgtcacg
      541 tattttgtac taatatcttt gtgtttacag tcaaattaat tccaattatc tctctaacag
      601 ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctcg gtgaaggggg
      661 cggccgctcg aggctagtct cgtgatcgat accgtcgaga tccgttcact aatcgacaat
      721 tgagtcttcg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      781 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      841 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      901 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      961 aaaagatgct gaa
//
