LOCUS       sequence-150229-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Indicator of caspase-3-like protease activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Zhang J, Wang X, Cui W, Wang W, Zhang H, Liu L, Zhang Z, Li Z, Ying 
            G, Zhang N, Li B
  TITLE     Visualization of caspase-3-like activity in cells using a 
            genetically encoded fluorescent biosensor activated by protein 
            cleavage.
  JOURNAL   Nat Commun. 2013;4:2157. doi: 10.1038/ncomms3157.
  PUBMED    23857461
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2157. doi"; pages: " 
            10.1038/ncomms3157"
COMMENT     Sequence Label: pCDH-puro-CMV-VC3AI
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     437..458
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(510..531)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(771..790)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag attctagagc tagcgaattg ccaccatgta cccctacgac gtgcccgact
      121 acgccatcaa gatcgccacc aggaagtacc tgggcaagca gaacgtgtac gacatcggcg
      181 tggagaggga ccacaacttc gccctgaaga acggcttcat cgccagcaac tgcttcaacg
      241 aattcatgga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
      301 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      361 ccgtgctgct gcccgacaac cactacctga gctaccagtc caaactgagc aaagacccca
      421 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcgacgagg
      481 tggacggctt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      541 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      601 agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg
      661 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
      721 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      781 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      841 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      901 acaacagcca caacgtcta
//
