LOCUS       sequence-150235-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses untagged human rhinovirus 3C protease in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Raran-Kurussi S, Waugh DS
  TITLE     A dual protease approach for expression and affinity purification of
            recombinant proteins.
  JOURNAL   Anal Biochem. 2016 Apr 19;504:30-37. doi: 10.1016/j.ab.2016.04.006.
  PUBMED    27105777
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2016-04-19"; volume: "504:30-37. doi"; pages: " 
            10.1016/j.ab.2016.04.006"
COMMENT     Sequence Label: pSRK2706
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..33
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(607..631)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             651..692
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             702..719
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggaatatcac aagtttgtac aaaaaagcag gctttaagaa ggagatatac atatgggacc
       61 aaacacagaa tttgcactat ccctgttaag gaaaaacata atgactataa caacctcaaa
      121 gggagagttc acagggttag gcatacatga tcgtgtctgt gtgataccca cacacgcaca
      181 gcctggtgat gatgtactag taaatggtca gaaaattaga gttaaggata agtacaaatt
      241 agtagatcca gagaacatta atctagagct tacagtgttg actttagata gaaatgaaaa
      301 attcagagat atcaggggat ttatatcaga agatctagaa ggtgtggatg ccactttggt
      361 agtacattca aataacttta ccaacactat cttagaagtt ggccctgtaa caatggcagg
      421 acttattaat ttgagtagca cccccactaa cagaatgatt cgttatgatt atgcaacaaa
      481 aactgggcag tgtggaggtg tgctgtgtgc tactggtaag atctttggta ttcatgttgg
      541 cggtaatgga agacaaggat tttcagctca acttaaaaaa caatattttg tagagaaaca
      601 ataataaccc agctttcttg tacaaagtgg tgatcaattc gaagcttgaa ggtaagccta
      661 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt
      721 gagtttgatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      781 gagca
//
