LOCUS       sequence-150236-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for expression of FnCpf1 crRNA. It contains BsmB1 
            site for cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim JS
  TITLE     Genome-wide analysis reveals specificities of Cpf1 endonucleases in 
            human cells.
  JOURNAL   Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609.
  PUBMED    27272384
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609."
COMMENT     Sequence Label: pU6-Fn-crRNA
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(379..396)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(630..649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      181 cttgtggaaa ggacgaaaca ccgaatttct actgttgtag attgagacgg gatccatccg
      241 tctccttttt tctagattcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
      301 actagttgtc ttcctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      361 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      421 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      481 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      541 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      601 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      661 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      721 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      781 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      841 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      901 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      961 gtggtggcct aactacggct acactagaa
//
