LOCUS       sequence-150237-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for expression of FnCpf1 crRNA. It contains BsmB1 
            site for cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim JS
  TITLE     Genome-wide analysis reveals specificities of Cpf1 endonucleases in 
            human cells.
  JOURNAL   Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609.
  PUBMED    27272384
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609."
COMMENT     Sequence Label: pU6-Fn-crRNA
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(404..652)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(462..481)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(632..652)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        767..871
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc aggaagacaa
      301 ctagtcaata atcaatgtca acgcgtatat ctggcccgta catcgcgaat ctagaaaaaa
      361 ggagacggat ggatcccgtc tcaatctaca acagtagaaa ttcggtgttt cgtcctttcc
      421 acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt
      481 ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc
      541 acgtattttg tactaatatc tttgtgttta cagtcaaatt aattccaatt atctctctaa
      601 cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcggtgaagg
      661 gggcggccgc tcgaggctag tctcgtgatc gataccgtcg agatccgttc actaatcgac
      721 aattgagtct tcgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      781 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      841 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      901 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      961 agtaaaagat
//
