LOCUS       sequence-150239-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses untagged human rhinovirus 3C protease in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Raran-Kurussi S, Waugh DS
  TITLE     A dual protease approach for expression and affinity purification of
            recombinant proteins.
  JOURNAL   Anal Biochem. 2016 Apr 19;504:30-37. doi: 10.1016/j.ab.2016.04.006.
  PUBMED    27105777
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2016-04-19"; volume: "504:30-37. doi"; pages: " 
            10.1016/j.ab.2016.04.006"
COMMENT     Sequence Label: pSRK2706
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..22)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(32..73)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(691..715)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    756..780
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(780..799)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(781..799)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcaatggtg atggtgatga tgaccggtac gcgtagaatc gagaccgagg agagggttag
       61 ggataggctt accttcaagc ttcgaattga tcaccacttt gtacaagaaa gctgggttat
      121 tattgtttct ctacaaaata ttgtttttta agttgagctg aaaatccttg tcttccatta
      181 ccgccaacat gaataccaaa gatcttacca gtagcacaca gcacacctcc acactgccca
      241 gtttttgttg cataatcata acgaatcatt ctgttagtgg gggtgctact caaattaata
      301 agtcctgcca ttgttacagg gccaacttct aagatagtgt tggtaaagtt atttgaatgt
      361 actaccaaag tggcatccac accttctaga tcttctgata taaatcccct gatatctctg
      421 aatttttcat ttctatctaa agtcaacact gtaagctcta gattaatgtt ctctggatct
      481 actaatttgt acttatcctt aactctaatt ttctgaccat ttactagtac atcatcacca
      541 ggctgtgcgt gtgtgggtat cacacagaca cgatcatgta tgcctaaccc tgtgaactct
      601 ccctttgagg ttgttatagt cattatgttt ttccttaaca gggatagtgc aaattctgtg
      661 tttggtccca tatgtatatc tccttcttaa agcctgcttt tttgtacaaa cttgtgatat
      721 tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc tcacaattcc
      781 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac gc
//
